Mouse GM-CSF
Mouse GM-CSF is a 14 kDa protein that is believed to contain 124 amino acid residues based on its cDNA sequence.
Evidence from several laboratories demonstrates that it is primarily produced by activated T cells and macrophages in mice. It is also produced by other cell types including endothelium and fibroblasts when stimulated by TNF, IL-1, IL-2, and IFN-γ. GM-CSF stimulates growth of macrophages, granulocytes, and dendritic cells and it plays a pivotal role in the immune response in mice.
There are membrane-bound and soluble forms of GM-CSF. Soluble GM-CSF is found in a complex associated with the extracellular matrix. Both forms function in the biological processes mentioned above. Recombinant GM-CSF synthesized in E. coli is nonglycosylated and is also biologically active.
2002
Format: 96-well strip plate
Assay range: 10-640 pg/ml
Sensitivity: 5 pg/ml
Total Assay Time: 3 hour and 45 minutes
Sample Size: 100 µl/well
Sample types: Serum, plasma and Cell Culture Supernatant
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