Mouse IL-2 is a T cell-derived cytokine known to promote growth and differentiation of T cells and to have pleiotropic effects on many other leukocytes.

IL-2 is rapidly secreted upon activation by T helper cells and certain subsets of memory cells. Mature mouse IL-2 is a 15.5 kDa protein containing 133 amino acid residues after several posttranslational processing. It is primarily produced by activated T and B cells and macrophages in mice. It is also produced by other cell types such as fibroblasts and CD24+ cells when stimulated by TNF-α, IL-1, IL-2 and IFN-γ. Mouse IL-2 stimulates growth of macrophages, granulocytes, dendritic cells and other granulose cells.

There are two kinds of IL-2, a membrane-bound form and a soluble form in a complex associated with the extracellular matrix. Both play biological functions based on research. In addition, recombinant IL-2 generated in E. coli is nonglycosylated but is biologically active.

Format: 96-well strip plate

Assay range: 16-1024 pg/ml

Sensitivity: 8 pg/ml

Total Assay Incubation Time: 3 hour and 45 minutes

Sample Size: 100 µl/well

Sample types: Serum, plasma and Cell Culture Supernatant

Wavelength for plate reading: A450 nm