is designed for the labeling of proteins with molecular weights greater than 25 kD (in particular antibodies), using a reactive succinimidyl-ester of N-methanthranilic acid (MANT).

The conjugates result from the formation of a stable covalent amide linkage.

The protein-dye conjugates have fluorescence-excitation and fluorescence-emission maxima at around 331 nm and 426 nm, respectively. Up to 50 nmol of protein can be labeled using one vial (1 µMol) of the reactive Fluoro•Spin 331 dye. In order to avoid unspecific interactions or unstable ester bond formation between dye and protein, this kit provides hydroxylamine for use as a stop reagent.

This kit includes enough reactive dye for up to 5 labeling reactions and 10 Centri•Sep spin columns for rapid and efficient purification of your protein-dye conjugates.

To get a quick start with IgG, use the Optimized Protocol for Labeling 1 mg of IgG or the Optimized Protocol for Labeling 100 µg of IgG (i.e. monoclonal antibodies).

For proteins other than IgG or to raise or lower the degree of labeling, please carefully and thoroughly read the instructions before starting.