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635 Protein Labeling Purification Kit
Fluoro•Spin 635 Protein Labeling & Purification Kit
is designed for the labeling of proteins with molecular weights greater than 25 kD (in particular antibodies), using a reactive succinimidyl-ester of DY-633 from DYOMICS.
The conjugates result from the formation of a stable covalent amide linkage.
The protein-dye conjugates have fluorescence-excitation and fluorescence-emission maxima at around 635 nm and 654 nm, respectively.
Up to 15 nmol of protein can be labeled using one vial (50 nMol) of the reactive Fluoro•Spin 635 dye.
In order to avoid unspecific interactions or unstable ester bond formation between dye and protein, this kit provides hydroxylamine for use as a stop reagent.
This kit includes enough reactive dye for up to 5 labeling reactions and 10 Centri•Sep spin columns for rapid and efficient purification of your protein-dye conjugates.
To get a quick start with IgG, use the Optimized Protocol for Labeling 1 mg of IgG or the Optimized Protocol for Labeling 100 µg of IgG (i.e. monoclonal antibodies).
For proteins other than IgG or to raise or lower the degree of labeling, please carefully and thoroughly read the instructions before starting.
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