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 HOME > Biolchemical Reagents > Biotins_Avidins |
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Biotin-4-fluorescein |
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코 드 |
: 3006 |
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단 위 |
: 5 mg |
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공 급 원 |
: ABD |
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가 격 |
: 문의 |
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다운로드 |
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Biotin-4-fluorescein
This bifunctional biotin conjugates demonstrates better binding and stronger fluorescence than biotin fluorescein. It has similar avidin-binding properties in terms of high affinity, fast association, and non-cooperative binding to avidin and streptavidin tetramers.
These exceptional properties are attributed to the small size/length of the new ligand since all larger/longer biotin derivatives are known for their mutual steric hindrance and anti-cooperative binding in 4:1 complexes with avidin and streptavidin tetramers. Specific binding of this biotin-fluorescein conjugate towards avidin and streptavidin is accompanied by 84-88% quenching of ligand fluorescence. It is used for the quantitation of biotin-binding sites.
Both the fluorescence and absorbance of biotin-4-fluorescein are quenched upon binding to one of the four biotin-binding sites of streptavidin, avidin, or the streptavidin conjugates of fluorescent dyes or enzymes. As a result, when a known concentration of biotin-4-fluorescein is added to a known amount of streptavidin, one can estimate the number of biotin-binding sites. |
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Biotin fluorescein
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Product Code |
Unit |
Price |
Availability |
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3006 |
5 mg |
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