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HOME > Functional Assays > Apoptosis Assay
Caspase 3/7 Activity Apoptosis Assay Kit
   
코    드 : 22796
단     위 : 200 assay
공 급 원 : ABD Bioquest
가     격 : 문의
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  Caspase 3/7 Apoptosis Assay
caspase 3/7 Activity Kit * Green Fluorescence *


This particular kit is designed to monitor cell apoptosis through measuring Caspase 3 activation. Caspase 3 is widely accepted as a reliable indicator for cell apoptosis since the activation of caspase-3 (CPP32/apopain) is important for the initiation of apoptosis. Caspase 3 has substrate selectivity for the peptide sequence Asp-Glu-Val-Asp (DEVD). This kit uses Z-DEVD-Rh 110-DVED-Z as a fluorogenic indicator for caspase-3 activity. Cleavage of Rh 110 peptides by caspase 3 generates strongly fluorescent Rh 110 that is monitored fluorimetrically at 520-530 nm with excitation of 480-500 nm. The kit provides all the essential components with an optimized assay protocol. Using 100 ul of reagents per well in a 96-well format, this kit provides sufficient reagents to perform 200 assays. Using 25 ul of reagents per well in a 384-well format, this kit provides sufficient reagents to perform 800 assays.


Key Features

Non-Radioactive : No special requirements for waste treatment.
Continuous : Easily adapted to automation with minimal hands on time.
Convenient : All essential assay components are included.
Optimized Performance : Optimal conditions for the detection of caspase 3 activity.
Enhanced value : Less expensive than the sum of individual components.

Kit Components

Component A : Caspase 3/7 Substrate (200X stock solution)          2 vials (50 μL/vial)
Component B : Assay buffer                                                       20 mL

Brief Summary

→ Prepare cells with test compounds (100 μL /96-well-plate or 25 μL 384-well-plate)
→ Add equal volume of caspase 3/7 assay solution (100 μL/96-well plate or 25 μL/384-well plate)
→ Incubate at room temperature for 1 hr
→ Read Fluorescence at Ex 494/Em 525

References

1. N. A. Thornberry and Y. Lazebnik, Science 281, 1312-1316 (1998).
2. J. C. Reed, J.Clin.Oncol. 17, 2941-2953 (1999).
3. Y. A. Lazebnik, S. H. Kaufmann, S. Desnoyers, G. G. Poirier, W. C. Earnshaw, Nature 371, 346-347 (1994).
4. P. Villa, S. H. Kaufmann, W. C. Earnshaw, Trends Biochem.Sci. 22, 388-393 (1997).
5. Y. Liu et al., Anal.Biochem. 267, 331-335 (1999).
6. M. Sakaue, Y. Motoyama, K. Yamamoto, T. Shiba, T. Teshima, K.Chiba. Biochem Biophys Res Commun, 350, 878 (2006)
7. T. Kume, R. Taguchi, H. Katsuki, M. Akao, H. Sugimoto, S. Kaneko, A. Akaike. Eur J Pharmacol, 542, 69 (2006) (2006)
8. M. Fennell, H. Chan , A Wood. J Biomol Screen, 11, 296 (2006)
9. X. Wu, J. Simone, D. Hewgill, R. Siegel, PE. Lipsky, L. He. Cytometry A, 69, 477 (2006)
   
 
  Caspase Assay


Product Code

Unit

Price

Availability

22796

200 assay