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HOME > Functional Assays > Apoptosis Assay
Mitochondrial Membrane Potential Assay Kit
   
코    드 : 22800
단     위 : 5 x 96 well plates
공 급 원 : ABD Bioquest
가     격 : 문의
다운로드

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  Mitochondria Assay
Cell Meter™ JC-10 Mitochondrial Membrane Potential Assay Kit


Although JC-1 is widely used in many labs, its poor water solubility makes it hard to use for some applications. Even at 1 µM concentration, JC-1 tends to precipitate in aqueous buffer. JC-10 has been developed to be a superior alternative to JC-1 where high dye concentration is desired. Compared to JC-1, our JC-10 has much better water solubility. JC-10 is capable of entering selectively into mitochondria, and changes reversibly its color from green to orange as membrane potentials increase. This property is due to the reversible formation of JC-10 aggregates upon membrane polarization that causes shifts in emitted light from 520 nm (i.e., emission of JC-10 monomeric form) to 570 nm (i.e., emission of J-aggregate). When excited at 490 nm, the color of JC-10 changes reversibly from green to greenish orange as the mitochondrial membrane becomes more polarized. This Cell Meter™ JC-10 Mitochondrial Membrane Potential Assay Kit enable you to monitor mitochondrial membrane potential changes using a simple microplate reader while all the other commercial JC-1 assay kits require the use of a flow cytometer. Our kit provides the most robust method to monitor mitochondrial membrane potential changes, and can be readily used for screening a large compound library.



JC-10 and JC-1 Comparison on Effect of Campotothecin induced mitochondria membrane potential change in JurKat cells. Jurkat cells were treated with 10 μM camptothecin for 4 hours. JC-1 and JC-10 dye loading solution was then added to the wells for 30 minutes. The fluorescent intensity for both J-aggregates and monomeric forms of JC-1 and JC-10 was measured with BMG NOVOstar microplate reader at Ex 485nm/Em 520 and 595nm.




Key Features

Increased Signal Intensity : Larger assay window
Increased solubility : Much better water solubility than JC-1
Convenient and Robust : Formulated to have minimal hands-on time
Versatile applications : Compatible with many cell lines and targets

Kit Components

Component A : 100X JC-10 in DMSO 1 vial (250 μL)
Component B : Assay buffer A 1 bottle (25 mL)
Component C : Assay buffer B 1 bottle (25 mL)

Brief Summary

→ Prepare cells
→ Add test compounds
→ Add JC-10 dye-loading solution (50 μL for 96-wellplate or 12.5 μL for 384-well-plate)
→ Incubate at room temperature for 1 h
→ Add Assay Buffer B (50 μL for 96-well-plate or 12.5 μL for 384-well-plate)
→ Read Fluorescence at Ex=490/Em=520 and 590nm

   
 
  JC-10 Mitochondrial Assay


Product Code

Unit

Price

Availability

22800

5 plates

 

22801

100 assay