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 HOME > Functional Assays > Apoptosis Assay |
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Mitochondrial Membrane Potential Assay Kit |
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코 드 |
: 22800 |
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단 위 |
: 5 x 96 well plates |
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공 급 원 |
: ABD Bioquest |
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가 격 |
: 문의 |
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다운로드 |
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Mitochondria Assay
Cell Meter™ JC-10 Mitochondrial Membrane Potential Assay Kit
Although JC-1 is widely used in many labs, its poor water solubility makes it hard to use for some applications. Even at 1 µM concentration, JC-1 tends to precipitate in aqueous buffer. JC-10 has been developed to be a superior alternative to JC-1 where high dye concentration is desired. Compared to JC-1, our JC-10 has much better water solubility. JC-10 is capable of entering selectively into mitochondria, and changes reversibly its color from green to orange as membrane potentials increase. This property is due to the reversible formation of JC-10 aggregates upon membrane polarization that causes shifts in emitted light from 520 nm (i.e., emission of JC-10 monomeric form) to 570 nm (i.e., emission of J-aggregate). When excited at 490 nm, the color of JC-10 changes reversibly from green to greenish orange as the mitochondrial membrane becomes more polarized. This Cell Meter™ JC-10 Mitochondrial Membrane Potential Assay Kit enable you to monitor mitochondrial membrane potential changes using a simple microplate reader while all the other commercial JC-1 assay kits require the use of a flow cytometer. Our kit provides the most robust method to monitor mitochondrial membrane potential changes, and can be readily used for screening a large compound library.
JC-10 and JC-1 Comparison on Effect of Campotothecin induced mitochondria membrane potential change in JurKat cells. Jurkat cells were treated with 10 μM camptothecin for 4 hours. JC-1 and JC-10 dye loading solution was then added to the wells for 30 minutes. The fluorescent intensity for both J-aggregates and monomeric forms of JC-1 and JC-10 was measured with BMG NOVOstar microplate reader at Ex 485nm/Em 520 and 595nm.
Key Features
Increased Signal Intensity : Larger assay window
Increased solubility : Much better water solubility than JC-1
Convenient and Robust : Formulated to have minimal hands-on time
Versatile applications : Compatible with many cell lines and targets
Kit Components
Component A : 100X JC-10 in DMSO 1 vial (250 μL)
Component B : Assay buffer A 1 bottle (25 mL)
Component C : Assay buffer B 1 bottle (25 mL)
Brief Summary
→ Prepare cells
→ Add test compounds
→ Add JC-10 dye-loading solution (50 μL for 96-wellplate or 12.5 μL for 384-well-plate)
→ Incubate at room temperature for 1 h
→ Add Assay Buffer B (50 μL for 96-well-plate or 12.5 μL for 384-well-plate)
→ Read Fluorescence at Ex=490/Em=520 and 590nm
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JC-10 Mitochondrial Assay
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Product Code |
Unit |
Price |
Availability |
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22800 |
5 plates |
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22801
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100 assay
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