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HOME > Functional Assays > Calcium Detection
Rhod-4 Calcium Assay Kit
   
코    드 : 36331
단     위 : 10 plates
공 급 원 : ABD Bioquest
가     격 : 문의
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  Calcium Assay Kit
Screen Quest™ Rhod-4 No Wash Calcium Assay Kit  *Medium Removal*


Calcium flux assays are preferred methods in drug discovery for screening G protein coupled receptors (GPCR). Screen Quest™ Rhod-4 NW Calcium Assay Kit provides a homogeneous fluorescence-based assay for detecting the intracellular calcium mobilization. Cells expressing a GPCR of interest that signals through calcium are pre-loaded with our proprietary Rhod-4 NW which can cross cell membrane.

Rhod-4 is the brightest red calcium indicator available for HTS screening. Once inside the cell, the lipophilic blocking groups of Quest Rhod-4™ are cleaved by non-specific cell esterase, resulting in a negatively charged fluorescent dye that stays inside cells, and its fluorescence is greatly enhanced upon binding to calcium. When cells stimulated with screening compounds, the receptor signals release of intracellular calcium, which greatly increase the fluorescence of Rhod-4. The characteristics of its long wavelength, high sensitivity, and >250 times fluorescence increases (when it forms complexes with calcium) make Quest Rhod-4™ an ideal indicator for measurement of cellular calcium.

This Screen Quest Rhod-4 NW Calcium Assay Kit provides an optimized assay method for monitoring G-protein-coupled receptors (GPCRs) and calcium channels. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation.


Key Features

Longer Wavelengths : Multiple excitations at 488, 514, 532 and 546 nm; Maximum emission at ~555 nm.
Larger Assay Window : 2 fold brighter, and 10 fold larger assay window than Rhod-2 AM.
Convenient and Robust : Formulated to have minimal hands-on time. No wash required.
Versatile applications : Compatible with many cell lines and receptors.

Kit Components

Component A : Rhod-4 NW                     1 vial, lyophilized
Component B : 10X Pluronic F127 Plus     10 bottles (1 ml/bottle)
Component C : HHBS                             1 bottle

Brief Summary

→ Prepare cells
→ Take medium off
→ Add Rhod-4 NW dye-loading solution (100 μL for 96- well-plate or 25 μL for 384-well-plate)
→ Incubate at room temperature for 1 hr
→ Read Fluorescence at Ex=530/Em=570
   
 
  Calcium detection

Product Code

Unit

Price

Availability

36331

10 plates