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HOME > Functional Assays > Calcium Detection
Fluo-8, AM
   
코    드 : 21080
단     위 : 1 mg
공 급 원 : AAT Bioquest
가     격 : 문의
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  Fluor8am
Quest Fluo-8™, AM


Storage : -20℃
Ex (nm) : 494           Em (nm) : 517
MW       : 1000          Solvent : DMSO

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Fluo-3 and Fluo-4 are most commonly used among the visible light-excitable calcium indicators. However, Fluo-3 AM and Fluo-4 AM are only moderately fluorescent in live cells upon esterase hydrolysis, and require harsh cell loading conditions to maximize their cellular calcium responses. Quest Fluo-8™ are developed to improve cell loading and calcium response while maintaining the convenient Fluo-3 and Fluo-4 spectral wavelength of maximum excitation @ ~490 nm and maximum emission @ ~520 nm. Quest Fluo-8™ AM only requires room temperature while Fluo-3 AM
and Fluo-4 AM require 37 oC cell loading. In addition, Quest Fluo-8™ is 2 times brighter than Fluo-4 AM, and 4 times brighter than Fluo-3 AM. ABD Bioquest offers a set of our outstanding Quest Fluo-8™ reagents with different calcium binding affinities (Quest Fluo-8: Kd = 389 nM; Quest Fluo-8H: Kd = 232 nM; Quest Fluo-8L: Kd = 1.86 µM). We also offer versatile packing sizes to meet your special needs, e.g., 1 mg; 10x50 µg; 20x50 µg; HTS packages with no additional packaging charge.
   
 
  Fluo-8 AM

Product Code

Unit

Price

Availability

21080

1 mg

 

21081

5x50ug

 

 

21082

10x50ug

 

 

21083

20x50ug