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HOME > Functional Assays > Calcium Detection |
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Fluo-3, AM |
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ÄÚ µå |
: 21011 |
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: 1 mg |
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: AAT Bioquest |
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°¡ °Ý |
: ¹®ÀÇ |
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Fluo-3, AM Fluo-3, AM * UltraPure grade 99% *
Storage : -20¡É MW : 1129.85 Ex (nm) / Em (nm) : 506 / 526 Solvent : DMSO
Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Fluo-3 and Rhod-2 are most commonly used among the visible light-excitable calcium indicators.
Fluo-3 indicators are widely used in flow cytometry and confocal laser-scanning microscopy. More recently, Fluo-3, AM has been extensively used in cell-based high-throughput screening assays for functional GPCR assays. Fluo-3 is essentially nonfluorescent unless bound to Ca2+ and exhibits a quantum yield at saturating Ca2+ of ~0.14 and a Kd for Ca2+ of 390 nM. |
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Fluo-3 indicator
Product Code |
Unit |
Price |
Availability |
21011 |
1 mg |
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21013
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20x50ug
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