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HOME > Functional Assays > Calcium Detection
Fluo-3, AM
   
코    드 : 21011
단     위 : 1 mg
공 급 원 : AAT Bioquest
가     격 : 문의
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  Fluo-3, AM
Fluo-3, AM * UltraPure grade 99% *


Storage : -20℃
MW : 1129.85
Ex (nm) / Em (nm) : 506 / 526
Solvent : DMSO


Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Fluo-3 and Rhod-2 are most commonly used among the visible light-excitable calcium indicators.

Fluo-3 indicators are widely used in flow cytometry and confocal laser-scanning microscopy. More recently, Fluo-3, AM has been extensively used in cell-based high-throughput screening assays for functional GPCR assays. Fluo-3 is essentially nonfluorescent unless bound to Ca2+ and exhibits a quantum yield at saturating Ca2+ of ~0.14 and a Kd for Ca2+ of 390 nM.
   
 
  Fluo-3 indicator

Product Code

Unit

Price

Availability

21011

1 mg

 

21013

20x50ug