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HOME > Cell Biology > Cell staining
DAPI
   
코    드 : 17510
단     위 : 10 mg
공 급 원 : ABD Bioquest
가     격 : 문의
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  DAPI
DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride]  *UltraPure grade*


Storage : -20℃
Ex (nm) / Em (nm) : 358 / 461
Solvent  : Water

Molecular Formula  : C16H17Cl2N5
Molecular Weight     : 350.25
CAS Number/Name : 28718-90-3 / 1H-Indole-6-carboximidamide, 2-[4-(aminoiminomethyl)phenyl]-,dihydrochloride


DAPI is a fluorescent stain that binds strongly to DNA. It is used extensively in fluorescence microscopy.
Since DAPI passes through an intact cell membrane, it can be used to stain live cells besides fixed cells. For fluorescence microscopy, DAPI is excited with ultraviolet light. When bound to double-stranded DNA its absorption maximum is at 358 nm and its emission maximum is at 461 nm. One drawback of DAPI is that its emission is fairly broad. DAPI also binds to RNA although it is not as strongly fluorescent as it binds to DNA. Its emission shifts to around 400 nm when bound to RNA.

DAPI's blue emission is convenient for multiplexing assays since there is very little fluorescence overlap between DAPI and green-fluorescent molecules like fluorescein and green fluorescent protein (GFP), or red-fluorescent stains like Texas Red. Besides labeling cell nuclei, DAPI is also used for the detection of mycoplasma or virus DNA in cell cultures.
   
 
  DNA stain

Product Code

Unit

Price

Availability

17510

10 mg