These chemiluminescent test kits contain the unique photoprotein Pholasin which emits light in the presence of free radicals and certain oxidants. The antioxidant capacity of the sample under test is expressed as the percentage reduction of peak luminescence of Pholasin (and/or the delay in time in which the peak luminescence is observed) during the course of the test in the presence and absence of the sample. The results can be expressed against other antioxidant standards or as ABEL-RAC™ (relatively antioxidant capacity) scores per mg or material being tested.

A chemiluminescent test for assessing antioxidant capacity of a sample to scavenge peroxynitrite formed by the reaction between superoxide and nitric oxide released from SIN-1. This assay is especially good for clinical studies aimed at assessing oxidative stress arising from peroxyl radical-mediated lipid peroxidation. It can also be used for assessing antioxidants in food, cosmetics and other media.

CONTENTS

Kit components sufficient for 100 x 200μL tests

A.1 x bottles Pholasin? (50μg: reconstitute to 5mL)

B.1 x 50mL Reconstitution & Assay Buffer for Peroxynitrite assay

C.2 x 12mg SIN-1 (mixed with an inert material)

D.1 x 25mL Water for dissolving SIN-1

E.1 x Vit E Analogue Standard: VEA Standard (reconstitute to 2mL)

F.1 x 96 well white microplates

• Quantify antioxidant activity


• A range of different antioxidant tests


• ABEL-RAC™ (Relative Antioxidant Capacity)


• ABEL-RAC™ scores on finished products


• ABEL-RAC™ scores per mg or µL sample


• ABEL-RAC™ scores per dose


• Test raw materials, ingredients & finished products


• Identify possible synergy between ingredients


• Optimise formulations


• Monitor processes during manufacture


• Confirm batch to batch uniformity


• Quantify shelf life of ingredients and products

Samples of serum or plasma of people and other animals are expressed as TAC (total antioxidant capacity) scores against a set of antioxidant standards of known concentration. These ABEL-TAC™ scores have knowndistribution ranges.

It is now possible to measure the antioxidant activity in a sample of blood taken from a mere finger prick to determine the TAC of individuals and classify their scores. These precise measurements make people aware of their antioxidant status, providing reassurance or enabling them to take steps to remedy a low value.