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HOME > Functional Assays > Apoptosis Assay
Lysyl Oxidase Assay Kit
   
코    드 : 15255
단     위 : 500 assay
공 급 원 : ABD Bioquest
가     격 : 문의
다운로드

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  Lysyl oxidase(LOX)
Amplite™  Fluorimetric Lysyl Oxidase Assay Kit   *Red Fluorescence*


Lysyl oxidase(LOX) is an extracellular enzyme that catalyzes formation of aldehydes from lysine residues in collagen and elastin precursors. These aldehydes are highly reactive, and undergo spontaneous chemical reactions with other lysyl oxidase-derived aldehyde residues, or with unmodified lysine residues.

This results in cross-linking collagen and elastin which is essential for stabilization of collagen fibrils and for the integrity and elasticity of mature elastin. Lysyl oxidase has been identified as a possible tumor suppressor. Lysyl oxidase activity in biological samples is traditionally and most reliably assessed by tritium release end-point assays using radiolabeled collagen or elastin substrates involving laborious vacuum distillation of the released tritiated water.

This kit offers a sensitive fluorescent assay for lysyl oxidase activity that utilizes 1,5-diaminopentane as substrate, and released hydrogen peroxide is detected using our Amplite™ HRP substrate in HRP-coupled reactions. This method allows the detection of sub ng/mL lysyl oxidase and is much more sensitive than the currently available fluorimetric assay for this enzyme activity. This method eliminates the interference that occurs in some biological samples and can be readily used to detect lysyl oxidase activity in cell culture experiments.

Key Features

Sensitive : The kit detect as low as 40 ng lysyl oxidase in solution.
Continuous : Easily adapted to automation with no separation required.
Convenient : Formulated to have minimal hands-on time. No wash is required.
Non-Radioactive : No special requirements for waste treatment.

Kit Components

Component A : Amplite™ HRP substrate (light sensitive) 1 vial
Component B : Assay Buffer 1 bottle (50 mL)
Component C : Horseradish Peroxidase 1 vial (50 units)
Component D : DMSO 1 vial (200 μL)

Brief Summary

→ Prepare assay reaction mixture (50 μL)
→ Add Lysyl oxidase standards or test samples (50 μL)
→ Incubate at 37oC for 10-30 min
→ Read fluorescence at Ex 570 nm/Em 590 nm

References

1. Lau YK, Gobin AM, West JL. (2006) Overexpression of lysyl oxidase to increase matrix crosslinking and improve
    tissue strength in dermal wound healing. Ann Biomed Eng, 34, 1239.
2. Thomassin L, Werneck CC, Broekelmann TJ, Gleyzal C, Hornstra IK, Mecham RP, Sommer P. (2005)
    The Pro-regions of lysyl oxidase and lysyl oxidase-like 1 are required for deposition onto elastic fibers. J Biol
     Chem, 280, 42848.
3. Maki JM, Sormunen R, Lippo S, Kaarteenaho-Wiik R, Soininen R, Myllyharju J. (2005) Lysyl oxidase is essential for
    normal development and function of the respiratory system and for the integrity of elastic and collagen fibers in
    various tissues. Am J Pathol, 167,      927.
4. Peinado H, Del Carmen Iglesias-de la Cruz M, Olmeda D, Csiszar K, Fong KS, Vega S, Nieto MA, Cano A,
    Portillo F. (2005) A molecular role for lysyl oxidase-like 2 enzyme in snail regulation and tumor progression.
    Embo J, 24, 3446.
5. Fogelgren B, Polgar N, Szauter KM, Ujfaludi Z, Laczko R, Fong KS, Csiszar K. (2005) Cellular fibronectin binds to
    lysyl oxidase with high affinity and is critical for its proteolytic activation. J Biol Chem, 280, 24690.
6. Farjanel J, Seve S, Borel A, Sommer P, Hulmes DJ. (2005) Inhibition of lysyl oxidase activity can delay phenotypic
    modulation of chondrocytes in two-dimensional culture. Osteoarthritis Cartilage, 13, 120.
   
 
  >Lysyl oxidase activity

Product Code

Unit

Price

Availability

15255

500 assay