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 HOME > Functional Assays > ALP Assay |
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Alkaline Phosphatase Assay Kit (yellow) |
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코 드 |
: 11950 |
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단 위 |
: 500 assay |
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공 급 원 |
: ABD Bioquest |
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가 격 |
: 문의 |
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Alkaline Phosphatase Assay
Amplite™ Colorimetric Alkaline Phosphatase Assay Kit *Yellow Color*
Alkaline phosphatase is a highly sensitive enzyme for ELISA, immuno-histochemical, Northern, Southern and Western blot applications. It is widely used in various biological assays (in particular, immunoassays) and ELISA-based diagnostics. This Amplite™ Alkaline Phosphatase Assay Kit uses pNPP, a chromogenic phosphatase substrate, to quantify alkaline phosphatase activity in solutions, in cell extracts, in live cells as well as on solid surfaces (such as PVDF membranes). The kit provides all the essential components with our optimized ‘mix and read’ assay protocol that is compatible with HTS liquid handling instruments.
Key Features
Optimized : Optimized conditions for detecting alkaline phosphatase activity.
Continuous : Easily adapted to automation with no separation required.
Convenient : Formulated to have minimal hands-on time. No wash is required.
Non-Radioactive : No special requirements for waste treatment.
Kit Components
Component A : pNPP (light sensitive) 1 vial
Component B : Assay buffer 1 bottle (25 mL)
Component C : Alkaline phosphatase standard 1 vial (lyophilized powder, 10 units)
Brief Summary
→ Prepare assay reaction mixture (50 μL)
→ Add alkaline phosphatase standards or test samples (50 μL)
→ Incubate at 37℃ for 5-30 min
→ Read absorbance at 400 nm
References
1. Zhu X, Jiang C. (2006) 8-Quinolyl phosphate as a substrate for the fluorimetric determination of alkaline
phosphatase. Clin Chim Acta.
2. Ali AT, Penny CB, Paiker JE, Psaras G, Ikram F, Crowther NJ. (2006) The effect of alkaline phosphatase inhibitors
on intracellular lipid accumulation in preadipocytes isolated from human mammary tissue.
Ann Clin Biochem, 43, 207.
3. Lee DH, Lim BS, Lee YK, Yang HC. (2006) Effects of hydrogen peroxide (H(2)O(2)) on alkaline phosphatase activity
and matrix mineralization of odontoblast and osteoblast cell lines. Cell Biol Toxicol, 22, 39.
4. Ali AT, Penny CB, Paiker JE, van Niekerk C, Smit A, Ferris WF, Crowther NJ. (2005) Alkaline phosphatase is
involved in the control of adipogenesis in the murine preadipocyte cell line, 3T3-L1. Clin Chim Acta, 354, 101.
5. Rieu JP, Ronzon F, Place C, Dekkiche F, Cross B, Roux B. (2004) Insertion of GPIanchored alkaline phosphatase
into supported membranes: a combined AFM and fluorescence microscopy study. Acta Biochim Pol, 51, 189.
6. Palermo C, Manduca P, Gazzerro E, Foppiani L, Segat D, Barreca A. (2004) Potentiating role of IGFBP-2 on
IGF-II-stimulated alkaline phosphatase activity in differentiating osteoblasts. Am J Physiol Endocrinol Metab, 286,
E648. |
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pNPP
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Product Code |
Unit |
Price |
Availability |
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11950 |
500 assay |
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