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 HOME > Functional Assays > ALP Assay |
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Alkaline Phosphatase Assay Kit (Luminescence) |
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코 드 |
: 11956 |
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단 위 |
: 100 assay |
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공 급 원 |
: ABD Bioquest |
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가 격 |
: 문의 |
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ALP assay Kit
Amplite™ Luminometric Alkaline Phosphatase Assay Kit *Luminescence*
ALP assay Kit uses a proprietary luminogenic phosphatase substrate, to quantify alkaline phosphatase activity in solutions, in cell extracts, in live cells as well as on solid surfaces (such as PVDF membranes). This proprietary phosphatase substrate generates a luminescent product that produces strong luminescence upon interaction with phosphatase. The kit provides all the essential components with our optimized ‘mix and read’ assay protocol that is compatible with HTS liquid handling instruments. It has extremely high sensitivity, and can be used for the assays that require demanding sensitivity.
Key Features
Optimized : Optimized conditions for detecting alkaline phosphatase activity.
Continuous : Easily adapted to automation with no separation required.
Convenient : Formulated to have minimal hands-on time. No wash is required.
Non-Radioactive : No special requirements for waste treatment.
Kit Components
Component A : Phosphatase Substrate 1 vial (lyophilized powder)
Component B : Reaction buffer 1 bottle (5 mL)
Component C : Alkaline phosphatase standard 1 vial (lyophilized powder, 10 units)
Component D : Assay buffer 1 bottle (5 ml)
Brief Summary
→ Prepare assay reaction mixture (50 μL)
→ Add alkaline phosphatase standards or test samples (50 μL)
→ Incubate at RT for 30-60 min
→ Read luminescence activity
References
1. Zhu X, Jiang C. (2006) 8-Quinolyl phosphate as a substrate for the fluorimetric determination of alkaline
phosphatase. Clin Chim Acta.
2. Ali AT, Penny CB, Paiker JE, Psaras G, Ikram F, Crowther NJ. (2006) The effect of alkaline phosphatase inhibitors
on intracellular lipid accumulation in preadipocytes isolated from human mammary tissue. Ann Clin Biochem,
43, 207.
3. Lee DH, Lim BS, Lee YK, Yang HC. (2006) Effects of hydrogen peroxide (H(2)O(2)) on alkaline phosphatase activity
and matrix mineralization of odontoblast and osteoblast cell lines. Cell Biol Toxicol, 22, 39.
4. Ali AT, Penny CB, Paiker JE, van Niekerk C, Smit A, Ferris WF, Crowther NJ. (2005) Alkaline phosphatase is
involved in the control of adipogenesis in the murine preadipocyte cell line, 3T3-L1. Clin Chim Acta, 354, 101.
5. Rieu JP, Ronzon F, Place C, Dekkiche F, Cross B, Roux B. (2004) Insertion of GPIanchored alkaline phosphatase
into supported membranes: a combined AFM and fluorescence microscopy study. Acta Biochim Pol, 51, 189.
6. Palermo C, Manduca P, Gazzerro E, Foppiani L, Segat D, Barreca A. (2004) Potentiating role of IGFBP-2 on
IGF-II-stimulated alkaline phosphatase activity in differentiating osteoblasts. Am J Physiol Endocrinol Metab,
286, E648. |
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ALP activity assay
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Product Code |
Unit |
Price |
Availability |
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11956 |
200 assay |
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