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Hydrogen peroxide
Amplite™ Fluorimetric Hydrogen Peroxide Assay Kit *Red Fluorescence*
Hydrogen peroxide (H2O2) is a reactive oxygen metabolic by-product that serves as a key regulator for a number of oxidative stress-related states. It is involved in a number of biological events that have been linked to asthma, atherosclerosis, diabetic vasculopathy, osteoporosis, a number of neurodegenerative diseases and Down's syndrome. Perhaps the most intriguing aspect of H2O2 biology is the recent report that antibodies have the capacity to convert molecular oxygen into hydrogen peroxide to contribute to the normal recognition and destruction processes of the immune system. Measurement of this reactive species will help to determine how oxidative stress modulates varied intracellular pathways. Amplite™ Hydrogen Peroxide Assay Kit uses our Amplite™ Red peroxidase substrate to quantify hydrogen peroxide in solutions, in cell extracts and in live cells. It can also be used to detect a variety of oxidase activities through enzyme-coupled reactions.
Key Features
Broad Application : Can be used for quantifying hydrogen peroxide in solutions, in cell extracts and in live cells; and
for detecting a variety of oxidase activities through enzyme-coupled reactions.
Sensitive : The kit detect as low as 10 picomoles of H2O2 in solution.
Continuous : Easily adapted to automation with no separation required.
Convenient : Formulated to have minimal hands-on time. No wash is required.
Non-Radioactive : No special requirements for waste treatment.
Kit Components
Component A : Amplite™ Red peroxidase substrate 1 vial
Component B : H2O2 1 vial (3% stabilized solution, 200 μL)
Component C : Assay Buffer 1 bottle (100 mL)
Component D : Horseradish Peroxidase 1 vial (20 units)
Component E : DMSO 1 vial (1 mL)
Brief Summary
→ Prepare H2O2 reaction mixture (50 μL)
→ Add H2O2 standards or test samples (50 μL)
→ Incubate at room temperature for 10-30 min
→ Read fluorescence at Ex 570 nm/Em 590 nm
References
1. Hoffmann, O. M., Becker, D., and Weber, J. R. (2007) J Cereb Blood Flow Metab.
2. Funk, R. S., and Krise, J. P. (2007) Mol Pharm 4, 154-9.
3. Krebs, B., Wiebelitz, A., Balitzki-Korte, B., Vassallo, N., Paluch, S., Mitteregger, G., Onodera, T.,Kretzschmar, H.
A., and Herms, J. (2007) J Neurochem 100, 358-67.
4. Yang, Y., Xu, S., An, L., and Chen, N. (2007) J Plant Physiol.
5. Lee, J. E., Kim, H., Jang, H., Cho, E. J., and Youn, H. D. (2007) J Neurochem.
6. Goth, L. (2006) Redox Rep 11, 281-2.
7. Laloi, C., Stachowiak, M., Pers-Kamczyc, E., Warzych, E., Murgia, I., and Apel, K. (2007) Proc Natl Acad Sci USA
104, 672-7.
8. Watts, R. J., Finn, D. D., Cutler, L. M., Schmidt, J. T., and Teel, A. L. (2006) J Contam Hydrol. |
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