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Hydrogen Peroxide Assay Kit
Amplite™ Fluorimetric Hydrogen Peroxide Assay Kit *Near Infrared Fluorescence*
This Amplite™ Hydrogen Peroxide Assay Kit uses our unique Amplite™ IR peroxidase substrate to quantify hydrogen peroxide in solutions, in cell extracts and in live cells. Amplite™ IR generates the fluorescence that is pH-independent from pH 4 to 10. Thus it is superior alternative to ADHP (Amplex Red™) for the detections that require low pH where ADHP (Amplex Red™) has reduced fluorescence. In addition, Amplite™ IR generates a product that has maximum absorption of 647 nm with maximum emission at 670 nm. This near infrared absorption and fluorescence minimize the assay background that is often caused by the autofluorescence of biological samples that rarely absorb light beyond 600 nm. It can also be used to detect a variety of oxidase activities through enzyme-coupled reactions.
Key Features
Broad Application : Can be used for quantifying hydrogen peroxide in solutions, in cell extracts and
in live cells; and for detecting a variety of oxidase activities through enzyme-coupled reactions.
Sensitive : The kit detect as low as 10 picomoles of H2O2 in solution.
Continuous : Easily adapted to automation with no separation required.
Convenient : Formulated to have minimal hands-on time. No wash is required.
Kit Components
Component A : Amplite™ IR peroxidase substrate 1 vial
Component B : H2O2 1 vial (3% stabilized solution, 200 μL)
Component C : Assay Buffer 1 bottle (100 mL)
Component D : Horseradish Peroxidase 1 vial (100 units)
Component E : DMSO 1 vial (1 mL)
Brief Summary
→ Prepare H2O2 reaction mixture (50 μL)
→ Add H2O2 standards or test samples (50 μL)
→ Incubate at room temperature for 10-30 min
→ Read fluorescence at Ex 645 nm/Em 670 nm
References
1. Hoffmann, O. M., Becker, D., and Weber, J. R. (2007) J Cereb Blood Flow Metab.
2. Funk, R. S., and Krise, J. P. (2007) Mol Pharm 4, 154-9.
3. Krebs, B., Wiebelitz, A., Balitzki-Korte, B., Vassallo, N., Paluch, S., Mitteregger, G., Onodera, T.,Kretzschmar, H.
A., and Herms, J. (2007) J Neurochem 100, 358-67.
4. Yang, Y., Xu, S., An, L., and Chen, N. (2007) J Plant Physiol.
5. Lee, J. E., Kim, H., Jang, H., Cho, E. J., and Youn, H. D. (2007) J Neurochem.
6. Goth, L. (2006) Redox Rep 11, 281-2.
7. Laloi, C., Stachowiak, M., Pers-Kamczyc, E., Warzych, E., Murgia, I., and Apel, K. (2007) Proc Natl Acad Sci USA
104, 672-7.
8. Watts, R. J., Finn, D. D., Cutler, L. M., Schmidt, J. T., and Teel, A. L. (2006) J Contam Hydrol. |
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