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 HOME > Cell Biology > Antioxidant_Oxidative stress |
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Peroxidase Assay Kit |
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코 드 |
: 11552 |
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단 위 |
: 500 assay |
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공 급 원 |
: AAT bioquest |
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가 격 |
: 문의 |
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다운로드 |
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Peroxidase Assay
Amplite™ Fluorimetric Peroxidase Assay Kit *Red Fluorescence*
Peroxidase is a small molecule (MW ~40 KD) that can usually be conjugated to an antibody in a 4:1 ratio. Due to its small size, it rarely causes steric hindrance problem with antibody/antigen complex formation. Peroxidase is inexpensive compared to other labeling enzymes. The major disadvantage associated with peroxidase is their low tolerance to many preservatives such as sodium azide that inactivates peroxidase activity even at low concentration. HRP conjugates are extensively used as secondary detection reagents in ELISAs, immuno-histochemical techniques and Northern, Southern and Western blot analyses. We offer this quick (10 min) HRP assay in a one-step, homogeneous, no wash assay system. The kit can be used for ELISAs, characterizing kinetics of enzyme reaction and high throughput screening of oxidase inhibitors, etc.
Key Features
Broad Application : Can be used for quantifying HRP activities in solutions and solid surfaces (e.g, ELISA)
Sensitive : The kit detect as low as 10 mU/mL of HRP in solution.
Continuous : Easily adapted to automation with no separation required.
Convenient : Formulated to have minimal hands-on time. No wash is required.
Non-Radioactive : No special requirements for waste treatment.
Kit Components
Component A : Amplite™ Red peroxidase substrate 1 vial
Component B : H2O2 1 vial (3% stabilized solution, 200 μL)
Component C : Assay Buffer 1 bottle (100 mL)
Component D : Horseradish Peroxidase 1 vial (20 units)
Component E : DMSO 1 vial (1 mL)
Brief Summary
→ Prepare peroxidase reaction mixture (50 μL)
→ Add peroxidase standards or test samples (50 μL)
→ Incubate at room temperature for 10-30 min
→ Read fluorescence at Ex 570 nm/Em 590 nm
References
1. Porstmann, B., Porstmann, T., Nugel, E. and Evers, U. (1985). Which of the commonly used marker enzymes gives
the best results in colorimetric and fluorimetric enzyme immunoassays: horseradish peroxidase, alkaline
phosphatase, ß-galactosidase? J. Immunol. Meth. 79, 27-37.
2. Wordinger, R.J., Miller, G.W. and Nicodemus, D.S. (1987). Manual of Immunoperoxidase Techniques, 2nd Edition.
Chicago: American Society of Clinical Pathologists Press, pp. 23-24.
3. Yolken, R.H. (1982). Enzyme immunoassays for the detection of infectious antigens in body fluids: current limitations
and future prospects. Rev. Infect. Dis. 4(1), 35-68.
4. Cordell, J.L., et al. (1984). Immunoenzymatic labeling of monoclonal antibodies using immune complexes of
alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes). J. Histochem. Cytochem. 32,
219-229.
5. Passey, R.B., et al. (1977). Evaluation and comparison of 10 glucose methods and the reference method
recommended in the proposed product class standard. Clin. Chem. 23(1), 131.
6. Hosoda, H., Takasaki, W., Tsukamoto, R. and Nambara, T. (1987). Sensitivity of steroid immunoassays.
Comparison of alkaline phosphatase, ß-galactosidase and horseradish peroxidase as labels in a colorimetric assay
system. Chem. Pharm. Bull. 35, 3336-3342.
7. Samoszuk, M.K., et al. (1989). Antibody, Immunoconjugates and Radiopharmaceuticals 2, 37-46. |
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11551,11552,11553,11559
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Product Code |
Unit |
Fluorescene |
Availability |
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11551 |
500 assay |
Blue |
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11552
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500 assay |
Red
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11553
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500 assay |
Near Infrared
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11559
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500 assay |
Luminometric
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