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HOME > Cell Biology > Cell labeling
Cell Labeling Kit (green)
   
코    드 : 22601
단     위 : 500 assay
공 급 원 : AAT Bioquest
가     격 : 문의
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  Cell Labeling
Cell Explorer™ Fixed Cell Labeling Kit  *Green Fluorescence*


Fluorescence imaging kits are a set of tools for labeling cells for fluorescence microscopic investigations of cell functions. The effective labeling of cells provides a powerful method for studying cellular events in a spatial and temporal context. This particular kit is designed to uniformly label fixed mammalian cells in green fluorescence for long term microscopic examination. The kit uses a proprietary green fluorescent dye that is more fluorescent upon bonding to cellular components. The fluorescent dye used in the kit is quite photostable so that the images can be repeatedly examined. The kit provides all the essential components with an optimized cell-labeling protocol. It is an excellent tool for preserving of fluorescent images of particular cells, and can also be used for fluorescence microscope demonstrations.


Kit Components

Component A : Stain It™ Green         1 vial
Component B : DMSO                      1 vial (200 μL)

Brief Summary

→ Prepare Samples (microplate wells)
→ Remove the liquid from the plate
→ Add 100 μL/well of Stain It™ Green solution
→ Stain the cells at RT for 10 min to hours
→ Wash the cells
→ Examine the specimen under microscope with Ex 495/Em 525

References

1. Wolff M, Wiedenmann J, Nienhaus GU, Valler M, Heilker R. (2006) Novel fluorescent proteins for high content
    screening. Drug Discov Today, 11, 1054.
2. Lee S, Howell BJ. (2006) High-content screening: emerging hardware and software technologies. Methods
    Enzymol, 414, 468.
3. Haasen D, Schnapp A, Valler MJ, Heilker R. (2006) G protein-coupled receptor internalization assays in the high-
   content screening format. Methods Enzymol, 414, 121
4. Hudson CC, Oakley RH, Sjaastad MD, Loomis CR. (2006) High-content screening of known G protein coupled
    receptors by arrestin translocation. Methods Enzymol, 414, 63.
5. Martinez ED, Dull AB, Beutler JA, Hager GL. (2006) High-content fluorescence-based screening for epigenetic
    modulators. Methods Enzymol, 414, 21.
6. Giuliano KA. (2007) Optimizing the integration of immunoreagents and fluorescent probes for multiplexed high
    content screening assays. Methods Mol Biol, 356, 189.
   
 
  fluorescent image labeling

Product Code

Unit

Price

Availability

22601

500 assay