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HOME > Cell Biology > Cell labeling
Live Cell Labeling Kit
   
코    드 : 22607
단     위 : 10 plates
공 급 원 : AAT Bioquest
가     격 : 문의
다운로드

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  Cell Labeling Kit
Cell Explorer™ Live Cell Labeling Kit  *Green Fluorescence*


This particular kit is designed to uniformly label live cells in green fluorescence. The kit uses non-fluorescent calcein AM that becomes strongly fluorescent upon entering into live cells. Calcein AM is a hydrophobic compound that easily permeates intact live cells. The hydrolysis of the non-fluorescent calcein AM by intracellular esterases generates the strongly fluorescent hydrophilic calcein that is well-retained in the cell cytoplasm. Cells grown in black-walled plates can be stained and quantified in less than two hours. It can be readily adapted for high-throughput assays in a wide variety of fluorescence platforms such as microplate assays, immunocytochemistry and flow cytometry.  It is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. The kit provides all the essential components with an optimized cell-labeling protocol. It is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells.


Key Features

Convenient : Formulated to have minimal hands-on time. It can be applied to a broad spectrum of samples.
Continuous : Easily adapted to automation with no separation protocols.

Kit Components

Component A : Calcein Green™                                     1 vial
Component B : 10X Assay Buffer                                   10 bottles (1 mL/bottle)
Component C : HHBS (Hanks’ buffer with 20 mM Hepes)  1 bottle (100 mL)

Brief Summary

→ Prepare cells in growth medium
→ Add Calcein Green™ working solution 100 μL/well for 96-well plates or 25 μL/well for a 384-well plates
→ Stain the cells at RT for 30 min to 2 hours
→ Examine the specimen under microscope at Ex/Em = 490/525 nm

References

1. Wolff M, Wiedenmann J, Nienhaus GU, Valler M, Heilker R. (2006) Novel fluorescent proteins for high-content
    screening. Drug Discov Today, 11, 1054.
2. Lee S, Howell BJ. (2006) High-content screening: emerging hardware and software technologies. Methods
    Enzymol, 414, 468.
3. Haasen D, Schnapp A, Valler MJ, Heilker R. (2006) G protein-coupled receptor internalization assays in the high-
    content screening format. Methods Enzymol, 414, 121
4. Hudson CC, Oakley RH, Sjaastad MD, Loomis CR. (2006) High-content screening of known G protein-coupled
    receptors by arrestin translocation. Methods Enzymol, 414, 63.
5. Martinez ED, Dull AB, Beutler JA, Hager GL. (2006) High-content fluorescence-based screening for epigenetic
    modulators. Methods Enzymol, 414, 21.
6. Giuliano KA. (2007) Optimizing the integration of immunoreagents and fluorescent probes for multiplexed high
    content screening assays. Methods Mol Biol, 356, 189.
   
 
  live Cell Labeling Kit

Product Code

Unit

Price

Availability

22607

10 plates