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HOME > Cell Biology > Cell labeling
Lysosomal Staining Kit (red)
   
코    드 : 22658
단     위 : 500 assay
공 급 원 : AAT Bioquest
가     격 : 문의
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  Imaging kit
Lysosomal Staining Kit  *Red Fluorescence*


Fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as membranes, lysosomes, mitochondria and nuclei etc. The selective labeling of live cell compartments provides a powerful method for studying cellular events in a spatial and temporal context.

This particular kit is designed to label lysosomes of live cells in red fluorescence. The kit uses a proprietary lysotropic dye that selectively accumulates in lysosomes probably vial the lysosome pH gradient. The lysotropic indicator is a hydrophobic compound that easily permeates intact live cells, and trapped in lysosomes after it gets into cells. Its fluorescence is significantly enhanced upon entering lysosomes. This key feature significantly increases its selectivity for lysosomes. The labeling protocol is robust, requiring minimal hands-on time. It can be readily adapted for a wide variety of fluorescence platforms such as microplate assays, immunocytochemistry and flow cytometry.

 It is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. The kit provides all the essential components with an optimized cell-labeling protocol. It is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells.


Kit Components

Component A : Lysolite™ Red                     100 μL (500 x DMSO stock solution)
Component B : Live cell staining buffer          50 mL

Brief Summary

→ Prepare cells
→ Add dye working solution
→ Incubate at 37°C for 1/2-2 h
→ Analyze under fluorescence microscope at Ex 545/Em 590 nm (Tritc filter set)

References

1. Hung, H; Deerinck, TJ; Ellisman, MH; and Spector, DL. (1994) In vivo analysis of the stability and transport of nuclear
    poly(A)+ RNA. J Cell Biol 126, 877-899.
2. Barasch J, Kiss B, Prince A, Saiman L, Gruenert D, al-Awqati Q. (1991) Defective acidification of intracellular
    organelles in cystic fibrosis. Nature 1991; 352:70-73.
3. Jiang, LW; Maher,VM; McCormick, JJ and Schindler, M. (1990) Alkalinization of the lysosomes is correlated with ras
    transformation of murine and human fibroblasts. J Biol Chem 265, 4775-4777.
4. Griffiths, G; Hoflack, B; Simons, K; Mellman, I; Kornfeld, S. (1988) The mannose 6-phosphate receptor and the
    biogenesis of  lysosomes. Cell. 12;52(3):329–341.
   
 
  Lysosomal Staining kit

Product Code

Unit

Price

Availability

22658

500 assay