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HOME > Biolchemical Reagents > Fluorescent Dyes
FAM Phosphoramidite
   
코    드 : 6028
단     위 : 100 µmole
공 급 원 : ABD Bioquest
가     격 : 문의
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  FAM Phosphoramidite
FAM Phosphoramidite


Storage : -20℃
Ex (nm) / Em (nm) : 494 / 522
Solvent : MeCN

Molecular Formula :
Molecular Weight : 1176.35
CAS Number/Name :


There are several ways of labeling an oligonucleotide with fluorescein. The choice of label is diversified further, depending on the spectral requirements. 5’-fluorescein-CE phosphoramidite, derived from the single isomer 6-carboxyfluorescein, 5’-hexachlorofluorescein-CE phosphoramidite (HEX) and 5’-tetrachlorofluorescein-CE phosphoramidite (TET) can all be used to efficiently label an oligonucleotide at the 5’-end. Labeling the 3’-end of an oligo with fluorescein can be achieved using 3’-Fluorescein CPGs. Standard cleavage and deprotection with ammonium hydroxide liberates the fluorescein-labeled oligo when using any of these supports.

6-FAM phosphoramidite [5'-Fluorescein phosphoramidite] / 100 µmole / product code #6017.

References :

1. Browne KA. (2005) Sequence-specific, self-reporting hairpin inversion probes. J Am Chem Soc, 127, 1989.
2. Kim SJ, Bang EK, Kwon HJ, Shim JS, Kim BH. (2004) Modified oligonucleotides containing lithocholic acid in their
backbones: their enhanced cellular uptake and their mimicking of hairpin structures. Chembiochem, 5, 1517.
3. Dobson N, McDowell DG, French DJ, Brown LJ, Mellor JM, Brown T. (2003) Synthesis of HyBeacons and dua
l-labelled probes containing 2'-fluorescent groups for use in genetic analysis. Chem Commun (Camb), 1234.
4. Kutyavin IV, Lokhov SG, Afonina IA, Dempcy R, Gall AA, Gorn VV, Lukhtanov E, Metcalf M, Mills A, Reed MW,
Sanders S, Shishkina I, Vermeulen NM. (2002) Reduced aggregation and improved specificity of G-rich
oligodeoxyribonucleotides containing pyrazolo[3,4-d]pyrimidine guanine bases. Nucleic Acids Res, 30, 4952.
5. Hill KW, Taunton-Rigby J, Carter JD, Kropp E, Vagle K, Pieken W, McGee DP, Husar GM, Leuck M, Anziano DJ,
Sebesta DP. (2001) Diels--Alder bioconjugation of dienemodified oligonucleotides. J Org Chem, 66, 5352.
6. Wu H, Skrzypczynski Z, Cornwell MJ, Aboleneen H. (2000) Identification of unexpected modifications of fluorescein-
labeled oligodeoxynucleotides by nuclease P1 digestion and mass
spectrometric techniques. Rapid Commun Mass Spectrom, 14, 26.
7. Hakala H, Virta P, Salo H, Lonnberg H. (1998) Simultaneous detection of several oligonucleotides by time-
resolved fluorometry: the use of a mixture of categorized microparticles in a sandwich type mixed-phase
hybridization assay.Nucleic Acids Res, 26, 5581.
8. Ramirez-Vick JE, Garcia AA, Lee J. (1998) Recovery of an oligonucleotide using silver ions immobilized on
paramagnetic particles. Prep Biochem Biotechnol, 28, 243.
9. Meyer KL, Hanna MM. (1996) Synthesis and characterization of a new 5-thiol-protected deoxyuridine
phosphoramidite for site-specific modification of DNA. Bioconjug Chem, 7, 401.
10. Hagmar P, Bailey M, Tong G, Haralambidis J, Sawyer WH, Davidson BE. (1995) Synthesis and characterisation of
fluorescent oligonucleotides. Effect of internal labelling on protein recognition. Biochim Biophys Acta, 1244, 259.
11. Lee SP, Censullo ML, Kim HG, Knutson JR, Han MK. (1995) Characterization of endonucleolytic activity of HIV-1
integrase using a fluorogenic substrate. Anal Biochem, 227, 295.
12. Nelson PS, Kent M, Muthini S. (1992) Oligonucleotide labeling methods. 3. Direct labeling of oligonucleotides
employing a novel, non-nucleosidic, 2-aminobutyl-1,3- propanediol backbone. Nucleic Acids Res, 20, 6253.
13. Schubert F, Cech D, Reinhardt R, Wiesner P. (1992) Fluorescent labelling of sequencing primers for automated
oligonucleotide synthesis. DNA Seq, 2, 273.
14. Theisen P, McCollum C, Andrus A. (1992) Fluorescent dye phosphoramidite labelling of oligonucleotides. Nucleic
Acids Symp Ser, 99.
   
 
  FAM Phosphoramidite

Product Code

Unit

Price

Availability

6028

100 µmole

 

6029

10 x100 umole

 

 

6030

1 g