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FAM Phosphoramidite
FAM Phosphoramidite
Storage : -20℃
Ex (nm) / Em (nm) : 494 / 522
Solvent : MeCN
Molecular Formula :
Molecular Weight : 1176.35
CAS Number/Name :
There are several ways of labeling an oligonucleotide with fluorescein. The choice of label is diversified further, depending on the spectral requirements. 5’-fluorescein-CE phosphoramidite, derived from the single isomer 6-carboxyfluorescein, 5’-hexachlorofluorescein-CE phosphoramidite (HEX) and 5’-tetrachlorofluorescein-CE phosphoramidite (TET) can all be used to efficiently label an oligonucleotide at the 5’-end. Labeling the 3’-end of an oligo with fluorescein can be achieved using 3’-Fluorescein CPGs. Standard cleavage and deprotection with ammonium hydroxide liberates the fluorescein-labeled oligo when using any of these supports.
6-FAM phosphoramidite [5'-Fluorescein phosphoramidite] / 100 µmole / product code #6017.
References :
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Acids Symp Ser, 99. |
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