|
|
| |
|
 |
|
| |
 HOME > Cell Biology > Metabolism |
|
|
|
Ethanol Quantitation Kit |
| |
|
|
|
|
|
 |
코 드 |
: 40001 |
|
|
단 위 |
: 200 assay |
|
|
공 급 원 |
: AAT bioquest |
|
|
가 격 |
: 문의 |
|
다운로드 |
|
|
|
|
|
|
|
| |
|
|
|
| |
 |
|
 |
| |
|
|
|
| |
 |
| |
Ethanol Kit
Amplite™ Ethanol Quantitation Kit
This non-radioactive ethanol assay kit is based on the oxidation of ethanol by alcohol oxidase. The etnanol kit uses our Amplite™ HRP substrate that makes the kit recordable in a dual mode, either fluorimetric (Ex/Em = 570nm/590 nm) or colorimetric readout (570 nm). The ethanol quantitation kit provides all the essential components with an optimized assay protocol. The ethanol assay is robust, sensitive, and can be readily adapted for high throughput assays in a wide variety of applications that require the measurement of ethanol. The assay can be completed within 30 minutes.
Key Features
Sensitive : Ethanol assy kit detects as low as 0.0003% Ethanol in solution.
Continuous : Easily adapted to automation with no separation required.
Convenient : Formulated to have minimal hands-on time. No wash is required.
Non-Radioactive : No special requirements for waste treatment.
Kit Components
Component A : Amplite™ Reagent (light sensitive) 1 vial
Component B : Assay Buffer 1 bottle (10 mL)
Component C : Ethanol Enzyme Mix (lyophilized) 1 vial
Component D : DMSO 1 vial (200 μL)
Component E : Ethanol Standard 0.5 mL
Brief Summary
→ Prepare assay reaction mixture (50 μL)
→ Add Ethanol standards or test samples (50 μL)
→ Incubate at room temperature for 5-30 min
→ Read fluorescence at Ex 540 nm/Em 590 nm
References
1. Peterson KP, Bowers C, Peterson CM. (1998) Prevalence of ethanol consumption may be higher in women than
men in a university health service population as determined by a biochemical marker: whole bloodassociated
acetaldehyde above the 99th percentile for teetotalers. J Addict Dis, 17, 13.
2. Chen HM, Peterson CM. (1994) Quantifying ethanol by high performance liquid chromatography with precolumn
enzymatic conversion and
derivatization with fluorimetric detection. Alcohol, 11, 577.
3. Linares P, Ruz J, De Castro MD, Valcarcel M. (1987) Enzymatic determination of ethanol in saliva by flow injection
analysis. J Pharm Biomed Anal, 5, 701.
4. Fernandez Gomez A, Ruz Polonio J, Luque de Castro MD, Valcarcel Cases M. (1985) Automatic enzymatic-
fluorimetric determination of ethanol in blood by flow injection analysis. Clin Chim Acta, 148, 131. |
| |
|
| |
 |
| |
Ethanol Assay kit
|
Product Code |
Unit |
Price |
Availability |
|
40001 |
200 assay |
|
|
|
| |
|
|
