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Glucose assay Kit
Amplite™ Glucose Quantitation Kit
Glucose, a monosaccharide, is the most important carbohydrate in biology. It is a source of energy and metabolic intermediate for cell growth. Glucose is one of the main products of photosynthesis and starts cellular respiration in both prokaryotes and eukaryotes. Glucose level is a key diagnostic parameter for many metabolic disorders.
This glucose assay kit provides a quick and sensitive method for the measurement of glucose in various biological samples (e.g., serum, plasma, body fluid, food, growth medium, etc.). The glucose assay kit uses our Amplite™ HRP substrate that making the kit recordable in a dual more, either fluorimetric (Ex/Em = 570nm/590 nm) or colorimetric readout (570 nm). The glucose kit provides all the essential components with an optimized assay protocol. The assay kit is robust, and can be readily adapted for high-throughput assays in a wide variety of applications that require the measurement of glucose. For example, the assay might be suitable for monitoring glucose level during fermentation and glucose feeding in protein expression processes. It might also be used for monitoring glucose transporters.
Key Features
Sensitive : Glucose assay kit detects as low as 3 uM D-glucose in solution.
Continuous : Easily adapted to automation with no separation required.
Convenient : Formulated to have minimal hands-on time. No wash is required.
Non-Radioactive : No special requirements for waste treatment.
Kit Components
Component A : Amplite™ Red (light-sensitive) 1 vial
Component B : Assay Buffer 1 bottle (50 mL)
Component C : Horseradish Peroxidase (HRP) 1 vial (10 units)
Component D : Glucose Oxidase 1 vial (100 units)
Component E : DMSO 1 vial (200 μL)
Brief Summary
→ Prepare assay reaction mixture (50 μL)
→ Add Glucose standards or test samples (50 μL)
→ Incubate at 37oC for 10-30 min
→ Read fluorescence at Ex 540 nm/Em 590 nm
References
1. Delva P, Degan M, Trettene M, Lechi A. (2006) Insulin and glucose mediate opposite intracellular ionized
magnesium variations in human lymphocytes. J Endocrinol, 190, 711.
2. Delva P, Degan M, Pastori C, Faccini G, Lechi A. (2002) Glucose-induced alterations of intracellular ionized
magnesium in human lymphocytes. Life Sci, 71, 2119.
3. Wang XT, Au SW, Lam VM, Engel PC. (2002) Recombinant human glucose-6-phosphate dehydrogenase
Evidence for a rapid-equilibrium random-order mechanism. Eur J Biochem, 269, 3417.
4. Leira F, Louzao MC, Vieites JM, Botana LM, Vieytes MR. (2002) Fluorescent microplate cell assay to measure
uptake and metabolism of glucose in normal human lung fibroblasts. Toxicol In Vitro, 16, 267.
5. Goi G, Bairati C, Burlina A, Massaccesi L, Monciotti C, Segalini G, Testa R, Lombardo A. (2000) Plasma
glycohydrolase levels in patients with type 1 diabetes at onset and in subjects undergoing an intravenous glucose
tolerance test. Metabolism, 49, 1352.
6. Manduteanu I, Voinea M, Serban G, Simionescu M. (1999) High glucose induces enhanced monocyte adhesion to
valvular endothelial cells via a mechanism involving ICAM-1, VCAM-1 and CD18. Endothelium, 6, 315.
7. Ruiz-Munoz LM, Vidal-Vanaclocha F, Lampreabe I. (1997) Enalaprilat inhibits hydrogen peroxide production by
murine mesangial cells exposed to high glucose concentrations. Nephrol Dial Transplant, 12, 456.
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