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Rabbit CRP kit
C-Reactive Protein, Rabbit Quantitation kit
Rabbit sera/plasma for testing are diluted to 1:1000 (suggested) and allowed to react with pneumococcal Cpolysaccharide coated on specially treated microwells. After appropriate incubation, the wells are washed to remove unreacted serum proteins, and a Biotin labeled goat anti-rabbit CRP-IgG is then added to react with and tag the antigen-antibody complexes. Following another incubation period, the wells are again washed to remove unreacted conjugate. Avidin labeled with peroxidase is then added. A urea peroxide substrate with TMB as chromogen is added to start color development. Development of a blue color indicates a positive reaction while negative reactions appear colorless or with a trace of blue. The reaction is interrupted with a stop solution that turns the blue positive reactions to yellow. Negative reactions remain colorless or with a hint of yellow. Color intensity (absorbance) is read at a wavelength of 450 nm on a spectrophotometer or ELISA reader. Semi-quantification of absorbance can be accomplished by the use of a standard curve generated by measuring two-fold dilutions of the standard provided.
Detection Range
C-Reactive Protein, Rabbit..................1.56 - 25 ng/mL
MATERIALS SUPPLIEDThe Helica™ C-Reactive Protein kit supplies sufficient materials for 96 determinations. 1. CRP ELISA microplate
2. Conjugate (100x) 0.13 mL
3. CRP Standard, 1.0ug/mL (100X), 0.13 mL
4. Wash Buffer, 1 packet
5. TMB Substrate, 12 mL
6. Stop Solution, 12 mL
References
1. Buduneli, E. et. al. Systemic Low-Dose Doxycycline and Alendronate Administration and Serum Interleukin-1Beta,
Osteocalcin, and C-Reactive Protein Levels in Rats. Journal of Periodontology 2005;76:1927-1933
2. Cai, X. et.al. The comparative study of Sprague-Dawley and Lewis rats in adjuvant-induced arthritis. Arch.
Pharmacol. 2006; 373:140-147
3. Chen, K. et. Induction of Leptin Resistance Through Direct Interaction of C-reactive Protein with Leptin. Nature
Medicine 2006;12:425 - 432 (Human CRP)
4. Cho W. C. et.al. Differential Expression of Proteins in Kidney, Eye, Aorta, and Serum of Diabetic and Non- diabetic
Rats. J. Cell. Biochem. 2006; 99: 256 - 268
5. Kalani, R., et.al. Effects of Caloric Restriction and Exercise on Age-Related, Chronic Inflammation Assessed by
C-Reactive Protein and Interleukin-6Effects of Caloric Restriction and Exercise on Age-Related, Chronic
Inflammation Assessed by C-Reactive Protein and Interleukin-6. Journal of Gerontology: 2006; 61A: 3:211-217
6. Ling, S. and F. Jabali. Effect of Early Phase Adjuvant Arthritis on Hepatic P450 Enzymes and Pharmacokinetics of
Verapamil: An Alternative Approach to the Use of An Animal Model for Inflammation for Pharmacokinetic Studies.
Drug Metabolism and Disposition 2005; 33:579-586
7. Ortiz, R. et. al. Urinary C-Reactive Protein Excretion Increases in Angiotensin II-Dependent Hypertensive Rats.
FASEB Journal 2006; 20: 470.16
8. Shagdarsuren, E. et al. Complement Activation in Angiotensin II-Induced Organ Damage. Circ Res. 2005; 97:716-724 |
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