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Primate CRP kit
C-Reactive Protein, Primate Quantitation kit
The Helica C-reactive protein assay is intended for the detection and quantification of C-reactive protein (CRP) in primate serum.
Primate sera for testing are diluted to 1:500 and allowed to react with pneumococcal C-polysaccharide coated on specially treated micro-wells. After appropriate incubation, the wells are washed to remove unreacted serum proteins, and an enzyme-labeled rabbit anti-human CRP (conjugate) is then added to react with and tag the antigen-antibody complexes. Following another incubation period, the wells are again washed to remove unreacted conjugate. A urea peroxide substrate with TMB as chromogen is added to start color development. Development of a blue color indicates a positive reaction while negative reactions appear colorless or with a trace of blue. The reaction is interrupted with a stop solution that turns the blue positive reactions to yellow. Negative reactions remain colorless or with a hint of yellow. Color intensity (absorbance) is read at a wavelength of 450nm on a spectrophotometer or ELISA reader. Semiquantification of absorbance can be accomplished by the use of a standard curve generated by measuring two-fold dilutions of the standard provided.
Quantitative ELISA-based test kits for rat C-reactive protein (rCRP), canine CRP (cCRP), human CRP (hsCRP), mouse CRP (mCRP), primate CRP (pCRP), and rabbit CRP (lCRP) are being offered to assist you in your research applications.
Detection Range
C-Reactive Protein, Primate................0.12 - 10 ng/mL
MATERIALS SUPPLIEDThe Helica™ C-Reactive Protein kit supplies sufficient materials for 96 determinations. 1. CRP ELISA microplate
2. Conjugate (100x) 0.13 mL
3. CRP Standard, 1.0ug/mL (100X), 0.13 mL
4. Wash Buffer, 1 packet
5. TMB Substrate, 12 mL
6. Stop Solution, 12 mL
References
1. Buduneli, E. et. al. Systemic Low-Dose Doxycycline and Alendronate Administration and Serum Interleukin-1Beta,
Osteocalcin, and C-Reactive Protein Levels in Rats. Journal of Periodontology 2005;76:1927-1933
2. Cai, X. et.al. The comparative study of Sprague-Dawley and Lewis rats in adjuvant-induced arthritis. Arch.
Pharmacol. 2006; 373:140-147
3. Chen, K. et. Induction of Leptin Resistance Through Direct Interaction of C-reactive Protein with Leptin. Nature
Medicine 2006;12:425 - 432 (Human CRP)
4. Cho W. C. et.al. Differential Expression of Proteins in Kidney, Eye, Aorta, and Serum of Diabetic and Non- diabetic
Rats. J. Cell. Biochem. 2006; 99: 256 - 268
5. Kalani, R., et.al. Effects of Caloric Restriction and Exercise on Age-Related, Chronic Inflammation Assessed by
C-Reactive Protein and Interleukin-6Effects of Caloric Restriction and Exercise on Age-Related, Chronic
Inflammation Assessed by C-Reactive Protein and Interleukin-6. Journal of Gerontology: 2006; 61A: 3:211-217
6. Ling, S. and F. Jabali. Effect of Early Phase Adjuvant Arthritis on Hepatic P450 Enzymes and Pharmacokinetics of
Verapamil: An Alternative Approach to the Use of An Animal Model for Inflammation for Pharmacokinetic Studies.
Drug Metabolism and Disposition 2005; 33:579-586
7. Ortiz, R. et. al. Urinary C-Reactive Protein Excretion Increases in Angiotensin II-Dependent Hypertensive Rats.
FASEB Journal 2006; 20: 470.16
8. Shagdarsuren, E. et al. Complement Activation in Angiotensin II-Induced Organ Damage. Circ Res. 2005; 97:716-724 |
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