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HOME > Biological toxin test > Marinetoxin
DSP Rapid Kit (Diarrhoeic Shellfish Poisoning Test)
   
코    드 : BT0101
단     위 : 96 Test
공 급 원 : SCETI K.K
가     격 : 문의
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  DSP toxin
Diarrhoeic Shellfish Poisoning (DSP) is caused by the ingestion of shell­fish contaminated by toxic dino-agellates.
The assay is based on the inhibition of the protein phosphatase (PP2A) by DSP toxins (okadaic acid and dinophysistoxin :OA and DTXs), which is the next generation assay principal to ELISA and mass spectrometry.
The assay is based on the inhibition of the protein phosphatase (PP2A) by DSP toxins (OA and DTXs). This principal is same as in-vivo toxicity process of DSP, so this assay measure not only DSP but also entire toxicity, that is ideal evaluation methods for DSP.
Minimum detection limit is 0.036ug/g, and minimum quantification limit is 0.056ug/g

Assay Principle & Procedure

PP2A can hydrolyze a colorless artificial substrate, p-nitrophenyl phosphate (p-NPP), and produces the yellow color of p-nitrophenol (p-NP) in the alkaline solution. The intensity of the color is proportional to the enzyme activity and the absorbance is measured at 405 nm. The concentration of DSP toxins in the sample is calculated from the standard curve produced using known concentrations of OA.

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  DSP ELISA
Format colorimetric phosphatase inhibition assay
Storage -20℃
Package 96wells
Sample types shellfish whole meat extract
Sample size 50μL for Assay
Sensitivity 0.1 μg/g
Range 0.1 - 2 ng/mL
lncubation

30 min


Product Code

Unit

Price

Availability

BT0101

96 test

BT0102

48 test