DSP Rapid Kit (Diarrhoeic Shellfish Poisoning Test),BT0101







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	DSP Rapid Kit (Diarrhoeic Shellfish Poisoning Test)


	DSP toxin Diarrhoeic Shellfish Poisoning (DSP) is caused by the ingestion of shellfish contaminated by toxic dino-agellates. The assay is based on the inhibition of the protein phosphatase (PP2A) by DSP toxins (okadaic acid and dinophysistoxin :OA and DTXs), which is the next generation assay principal to ELISA and mass spectrometry. The assay is based on the inhibition of the protein phosphatase (PP2A) by DSP toxins (OA and DTXs). This principal is same as in-vivo toxicity process of DSP, so this assay measure not only DSP but also entire toxicity, that is ideal evaluation methods for DSP. Minimum detection limit is 0.036ug/g, and minimum quantification limit is 0.056ug/g Assay Principle & Procedure PP2A can hydrolyze a colorless artificial substrate, p-nitrophenyl phosphate (p-NPP), and produces the yellow color of p-nitrophenol (p-NP) in the alkaline solution. The intensity of the color is proportional to the enzyme activity and the absorbance is measured at 405 nm. The concentration of DSP toxins in the sample is calculated from the standard curve produced using known concentrations of OA.

DSP ELISA

Format	colorimetric phosphatase inhibition assay
Storage	-20℃
Package	96wells
Sample types	shellfish whole meat extract
Sample size	50μL for Assay
Sensitivity	0.1 μg/g
Range	0.1 - 2 ng/mL
lncubation	30 min

Product Code	Unit	Price	Availability
BT0101	96 test
BT0102	48 test