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HOME > Cell Biology > Cell labeling
F-Actin Labeling Kit
   
코    드 : 22663
단     위 : 500 assay
공 급 원 : AAT Bioquest
가     격 : 문의
다운로드

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  F-Actin Labeling kit
Cell Navigator™ F-Actin Labeling Kit *Orange Fluorescence*


Our Cell Navigator™ fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as membranes, lysosomes, mitochondria and nuclei etc. The selective labeling of live cell compartments provides a powerful method for studying cellular events in a spatial and temporal context. This particular kit is designed to label F-actins of fixed cells in red fluorescence. The kit uses a blue fluorescent phalloidin conjugate that is selectively bound to F-actins. This red fluorescent phalloidin conjugate is a high-affinity probe for F-actins. Used at nanomolar concentrations, phallotoxins are convenient probes for labeling, identifying and quantitating F-actins in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. The labeling protocol is robust, requiring minimal hands-on time. The kit provides all the essential components with an optimized staining protocol.


Kit Components

Component A : iFluor™ 546-Phalloidin             1 vial
Component B : Labeling Buffer                       50 mL
Component C : Methanol                                1 vial (1.5 mL)

Brief Summary

→ Prepare samples (microplate wells)
→ Remove the liquid from the plate
→ Add 100 μL/well of iFluor™ 546-Phalloidin solution
→ Stain the cells at RT for 15 to 60 min
→ Wash the cells
→  Examine the specimen under microscope at Ex/Em = 540/590 nm

References

1. Szczesna D, Lehrer SS (1993). The binding of fluorescent phallotoxins to actin in myofibrils. J Muscle Res Cell
    Motil, 14(6), 594.
2. Johnson S C, Nancy M. McKenna M N, and Wang Y (1988). Association of microinjected myosin and its
    subfragments with myofibrils in living muscle cells. J Cell Biol, 107(6), 2213.
3. Wang K, Feramisco JR, Ash JF (1982). Fluorescent localization of contractile proteins in tissue culture cells.
    Methods Enzymol, 85 Pt B, 514.
4. Miki M, Barden JA, dos Remedios CG, Phillips L, Hambly BD (1987). Interaction of phalloidin with chemically
    modified actin. Eur J Biochem 165, 125.5. Cooper JA. (1987). Effects of cytochalasin and phalloidin on actin.
    J Cell Biol 105, 1473.
   
 
  22660,22661,22663,22664

Product Code

Unit

Fluorescene

Availability

22660

500 assay

Blue

22661

500 assay

green

 

22663

500 assay

 Orange

 

22664

500 assay

 Red