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HOME > Functional Assays > Apoptosis Assay
Intracellular GSH Assay Kit
   
코    드 : 22810
단     위 : 100 assay
공 급 원 : AAT Bioquest
가     격 : 문의
다운로드

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  GSH Assay Kit
Cell Meter™ Intracellular GSH Assay Kit


Used for monitoring cell apoptosis. This particular kit is designed to detect cell apoptosis by measuring the decrease in reduced glutathione (GSH). GSH is important for maintaining redox level of cells. It is involved in many cellular processes including the scavenging of free radicals, drug detoxification, cell signaling, and cell proliferation. The decrease in cellular GSH concentration is an early hallmark in the progression of cell death in response to different apoptotic stimuli in many cells.
Our Cell Meter™ Intracellular GSH Assay Kit uses our proprietary non-fluorescent Thiolite™ Green, which becomes strongly fluorescent upon reacting with thiol (including GSH in cells). In normal cells, Thiolite™ Green is accumulated primarily in cytosol, but it is partially translocated to mitochondria in apoptotic cells while Thiolite™ Green staining intensity is decreased. Cells stained with Thiolite™ Green can be visualized with a flow cytometer at Ex/Em = 490/520 nm (FL1 channel). The kit can be used together with other reagents, such as 7-AAD (#17501) for multi-parametric study of cell viability and apoptosis.


Key Features

Optimized for screening apoptosis activators and inhibitors with a flow cytometer.

Kit Components

Component A : Thiolite™ Green                    1 vial
Component B : Assay Buffer                         1 bottle (100 mL)
Component C : DMSO                                  1 vial    (500 uL)

Brief Summary

→ Prepare cells with test compounds at a density of 5 × 105 to 1 × 106 cells/mL
→ Add 5 μL of 200X Thiolite™ Green into 1 mL of cell solution
→ Incubate at room temperature for 15-30 minute
→ Pellet the cells and resuspend the cells in 1 mL of growth medium
→ Analyze with a flow cytometer using the FL1 channel
   
 
  22810

Product Code

Unit

Price

Availability

22810

100 assay