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HOME > Cell Biology > Antioxidant_Oxidative stress
SOD Assay Kit
   
코    드 : 11305
단     위 : 100 T
공 급 원 : AAT bioquest
가     격 : 문의
다운로드

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  sod assay kit
Amplite™ Colorimetric Superoxide Dismutase Assay Kit * blue color *


Superoxide dismutases (SOD) are a class of enzymes that catalyze the dismutation of superoxide into oxygen and hydrogen peroxide. Superoxide is one of the main reactive oxygen species in cells. It is associated with neurodegenerative diseases, ischemia reperfusion injury, atherosclerosis and aging. SODs are an important antioxidant defense in nearly all cells exposed to superoxide radicals. It was reported that mice lacking SOD1 developed a wide range of pathologies, including hepatocellular carcinoma, an acceleration of age-related muscle mass loss, an earlier incidence of cataracts and a reduced lifespan. Overexpression of SOD protects murine fibrosarcoma cells from apoptosis and promotes cell differentiation.


Kit Components

Component A : ReadiView™ SOD560              2 vials
Component B : 50X Xanthine                         1 vial (100 μL)
Component C : Xanthine Oxidase                  2 vials
Component D : SOD Standard                        1 vial (500 Units)


Brief Summary

→ SOD standards or test samples (50 μL)
→ Add SOD Assay Mixture (25 μL)
→ Add Enzyme reaction mixture (25 μL)
→ Incubate at room temperature for 10-30 min
→ Read absorbance at 560 nm


References

1. Kussmaul L, Hirst J., The mechanism of superoxide production by NADH: ubiquinone oxidoreductase (complex I)
    from bovine heart mitochondria. PNAS 103: 7607-12, 2006.
2. Passos JF, Von Zglinicki T., Oxygen free radicals in cell senescence: are they signal transducers
    Free Radic Res 40:1277-83, 2006.
3. Szeto HH., Mitochondria-targeted peptide antioxidants: novel neuroprotective agents. AAPS J 8:E521-31, 2006.
4. Robak J, Gryglewski RJ. Flavonoids are scavengers of superoxide anions. Biochem Pharmacol 37:837-41, 1988.
5. Nebot C, Moutet M, Huet P, Xu J-Z, Yadan J-C, Chaudiere J., Spectrophotometric assay of superoxide dismutase
    activity based on the activated auto-oxidation of a tetracyclic catechol. Anal biochem 214:442-51, 1993.
   
 
  Sod assy kit

Product Code

Unit

Price

Availability

11305

100 assay