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cal 590 am
Cal-590™, AM
Storage : -20℃
Ex (nm) : 558 Em (nm) : 584
Solvent : DMSO
Cal-520™, Cal-590™ and Cal-630™ provide the most robust homogeneous fluorescence-based assay tools for detecting intracellular calcium mobilization. They are fluorogenic calcium-sensitive dyes with a significantly improved signal to noise ratio and intracellular retention compared to the existing calcium indicators (such as Fluo-3 AM, Fluo-4 AM and Rhod-2 AM). Cells expressing a GPCR or calcium channel of interest that signals through calcium can be preloaded with Cal-520™ AM, Cal-590™ AM or Cal-630™ AM which can cross cell membrane. Once inside the cell, the lipophilic blocking groups of Cal 520™ AM, Cal-590™ AM or Cal-630™ AM are cleaved by intracellular esterases, resulting in a negatively charged fluorescent dye that stays inside cells. Their fluorescence is greatly enhanced upon binding to calcium. When cells stimulated with agonists, the receptor signals the release of intracellular calcium, which significantly increase the fluorescence of Cal-520™, Cal-590™ or Cal-630™. The characteristics of high sensitivity and >100 times fluorescence enhancement make Cal-520™ AM, Cal-590™ AM or Cal-630™ AM ideal indicators for the measurement of cellular calcium. The high S/N ratio and better intracellular retention make the Cal-520™, Cal-590™ or Cal-630™ calcium assay a robust tool for evaluating GPCR and calcium channel targets as well as for screening their agonists and antagonists.Besides their convenient excitation wavelengths and large fluorescence enhancement by calcium, Cal-520™, Cal-590™, and Cal-630™ are predominantly localized in cytosols unlike Rhod-2 that is mainly localized in mitochondria. In addition, the long Ex/Em wavelengths of Cal-590™ and Cal-630™ make these dyes perfect calcium indicators compatible for multicolor detection with green fluorescent protein (GFP) cell lines. In addition, Cal-520™, Cal-590™ or Cal-630™ calcium assays are optimized to be compatible with most of the existing fluorescence instruments. Cal-520 can be well excited at 488 nm, and used with FITC filter set. Cal-590 is optimized to be excited at 555 nm, and used with TRITC/Cy3 filter set. Cal-590 is optimized to be excited at 594 nm, and used with Texas Red? filter set. |
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