홈으로 홈으로Contact Usenglish
   
 
  Protein Research
  Cell Biology
  Gel Filtration
  Biological toxin test
  DNA Isolation and Purification
  Cytokine Assays
  Biolchemical Reagents
  Functional Assays
  HORIBA 수질측정기
  Vortexing . 미생물
  UV INSTRUMENT
 
 
 
   
 
 
HOME > Cell Biology > Cell signaling
NADP/NADPH Assay Kit
   
코    드 : 15260
단     위 : 400 assay
공 급 원 : AAT Bioquest
가     격 : 문의
다운로드

:

 
       
   
       
 
  NADP/NADPH Assay
Amplite™ Colorimetric NADP/NADPH Assay Kit *Blue Color*



Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells. NADH is the reduced form of NAD+, and NAD+ is the oxidized form of NADH. It forms NADP with the addition of a phosphate group to the 2' position of the adenyl nucleotide through an ester linkage. NADP is used in anabolic biological reactions, such as fatty acid and nucleic acid synthesis, which require NADPH as a reducing agent. In chloroplasts, NADP is an oxidizing agent important in the preliminary reactions of photosynthesis. The NADPH produced by photosynthesis is then used as reducing power for the biosynthetic reactions in the Calvin cycle of photosynthesis. The traditional NAD/NADH and NADP/NADPH assays are done by monitoring of NADH or NADPH absorption at 340 nm. This method suffers low sensitivity and high interference since the assay is done in the UV range that requires expensive quartz microplate. This Amplite™ NADP/NADPH Assay Kit provides a convenient method for sensitive detection of NADP, NADPH and their ratio. The enzymes in the system specifically recognize NADP/NADPH in an enzyme cycling reaction. There is no need to purify NADP/NADPH from sample mix. The enzyme cycling reaction significantly increases detection sensitivity.


Key Features

Broad Application: Can be used for quantifying NADP/NADPH in solutions, in cell extracts.
Sensitive: The kit detect as low as 3 picomoles of NADP/NADPH in solution.
Continuous: Easily adapted to automation with no separation required.
Convenient: Formulated to have minimal hands-on time. No wash is required.
Non-Radioactive: No special requirements for waste treatment.

Kit Components

Component A: NADP/NADPH recycling enzyme mixture 2 bottles (lyophilized powder)
Component B: NADPH sensor buffer 1 bottle (20 mL)
Component C: NADPH standard (FW: 833.36) 1 vial (167 μg)

Brief Summary

→ Prepare NADP/NADPH reaction mixture (50 μL)
→ Add NADPH standards or test samples (50 μL)
→ Incubate at room temperature for 15 min-2hr
→ Read absorbance at 575±5 nm

References

1. Hedeskov CJ, Capito K, Thams P. (1987) Cytosolic ratios of free [NADPH]/[NADP+] and [NADH]/[NAD+] in mouse     pancreatic islets, and nutrient-induced insulin secretion. Biochem J, 241,161.
2. Gaetani GF, Ferraris AM, Sanna P, Kirkman HN. (2005) A novel NADPH:(bound) NADP+ reductase and
    NADH:(bound) NADP+ transhydrogenase function in bovine liver catalase. Biochem J, 385, 763.
3. Kobayashi K, Miura S, Miki M, Ichikawa Y, Tagawa S. (1995) Interaction of NADPH-adrenodoxin reductase with     NADP+ as studied by pulse radiolysis. Biochemistry, 34, 12932.
4. Marino D, Gonzalez EM, Frendo P, Puppo A, Arrese-Igor C. (2006) NADPH recycling systems in oxidative stressed     pea nodules: a key role for the NADP(+)-dependent isocitrate dehydrogenase. Planta.
   
 
  15260

Product Code

Unit

Price

Availability

15260

400 assay