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 HOME > Cell Biology > Cell signaling |
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Pyrophosphate Assay Kit |
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코 드 |
: 21611 |
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단 위 |
: 200 assay |
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공 급 원 |
: AAT Bioquest |
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가 격 |
: 문의 |
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다운로드 |
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Pyrophosphate Assay
PhosphoWorks™ Fluorimetric Pyrophosphate Assay Kit *Blue Fluorescence*
Pyrophosphate (PPi) are produced by a number of biochemical reactions, such as ATP hydrolysis, DNA and RNA polymerizations, cyclic AMP formation by the enzyme adenylate cyclase and the enzymatic activation of fatty acids to form their coenzyme A esters. Our PhosphoWroks™ Pyrophosphate Assay Kit provides the most robust spectrophotometric method for measuring pyrophosphate. This kit uses our proprietary fluorogenic pyrophosphate sensor that has its fluorescence intensity proportionally dependent upon the concentration of pyrophosphate. Our assay is much easier and more robust than the enzyme-coupling pyrophosphate methods that require at least two enzymes for their pyrophosphate detections. The kit provides all the essential components for assaying pyrophosphate. This kit has been successfully used in high throughput screening (HTS). Please inquire special HTS bulk package discount for the screening of >10,000 assays.
Kit Features
Universal : Can be used for monitoring any biological processes that generate pyrophosphate.
Continuous : Easily adapted to automation with no mixing or separation.
Convenient : Formulated to have minimal hands-on time.
Non-Radioactive : No special requirements for waste treatment.
Kit Components
Component A : Assay buffer 1 bottle (25 mL)
Component B : PPi senor 1 vial (lyophilized powder)
Component C : Pyrophasphate standard 1 mL (50 mM)
Component D : DMSO 1 vial (200 μL)
Brief Summary
→ Prepare test samples (50 μL) and pyrophosphate standards (50 μL)
→ Add Assay solution (50 μL)
→ Incubate at room temperature for 10 to 30 min
→ Read Fluorescence at Ex/Em = 316/456nm
References
1. Zhou M, Diwu Z, Panchuk-Voloshina N and Haugland RP. (1997) A Stable Nonfluorescent Derivative of Resorufin for
the Fluorometric Determination of Trace Hydrogen Peroxide: Applications in Detecting the Activity of Phagocyte
NADPH Oxidase and Other Oxidases Anal Biochem 253, 162-168.
2. Mohanty, JG, Jaffe JS, Schulman E S,and RaibleDG. (1997) A highly sensitive fluorescent microassay of H2O2
release from activated human leukocytes using a dihydroxyphenoxazine derivative. J. Immunol. Methods 202:
133-141. |
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Pyrophosphate
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Product Code |
Unit |
Price |
Availability |
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21611 |
200 assay |
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