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 HOME > Cell Biology > Antioxidant_Oxidative stress |
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Thiol (GSH) Quantitation Assay Kit |
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코 드 |
: 5524 |
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단 위 |
: 200 assay |
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공 급 원 |
: ABD bioquest |
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가 격 |
: 문의 |
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다운로드 |
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oxidized glutathione
Amplite™ Fluorimetric Thiol Quantitation Assay Kit *Green Fluorescence*
The detection and measurement of free thiol (such as free cysteine, glutathione and cysteine residues in proteins) is one of the essential tasks for investigating biological processes and events in many biological systems. The monitoring of reduced (GSH) and oxidized glutathione in biological samples is essential for evaluating the redox and detoxification status of cells and tissues in relation to the protective role of glutathione against oxidative and free-radical-mediated cell injury. Disorders of cysteine metabolism include cystinosis, an autosomal recessive disease produced by a defect in lysosomal transport, and cystinuria, a common heritable disorder of amino acid transport. There are few reagents or assay kits available for quantitating thiols in biological systems. However, all the commercial kits either lack sensitivity or have tedious protocols. Our Amplite™ Fluorimetric Thiol Qutitation Kit provides an ultrasensitive fluorimetric assay for quantitating thiols that exist either in a small molecule or on a protein. The kit uses a proprietary non-fluorescent dye that becomes strongly fluorescent upon reacting with thiol. The kit provides a sensitive, one-step fluorimetric method to detect as little as 1 picomole of cysteine or GSH in a 100 µL assay volume (10 nM in concentration). The assay is rapid and robust. It can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation with Ex/Em = 490 nm/520 nm.
Key Features
Broad Application : Can be used for quantifying thiol and sulfide in a variety of biological systems
(e.g., plasma, urine and cell extracts)
Sensitive : Detect as low as 1 picomole of thiol.
Continuous : Easily adapted to automation with no separation required.
Convenient : Formulated to have minimal hands-on time. No wash is required.
Non-Radioactive : No special requirements for waste treatment.
Kit Components
Component A : Thiolite Green™ 1 vial
Component B : Assay Buffer 1 bottle (25 ml)
Component C : GSH standard 1 vial (62 μg)
Component D : DMSO 1 vial (100 μL)
Brief Summary
→ Prepare Thiolite Green™ reaction mixture (50 μL)
→ Add GSH standards or test samples (50 μL)
→ Incubate at room temperature for 10 min-1 h
→ Read fluorescence at Ex 490 nm/Em 520nm nm
References
1. Mudd SH, Levy HL, Skovby F. Disorder of transsulfuration. In:Scriver CR, Beaudet AL, Sly WS,Valle D, eds.
The metabolic andmolecular bases of inherited disease, 7th ed. New York: McGraw-Hill, 1995:1229–1327.
2. Meister A. Selective modification of glutathione metabolism. Science 1983;220:472–7.
3. Gahl WA, Bashan N, Tietze F, Bernardini I, Schulman JD. Lysosomal cystine transport is defective in cystinosis.
Science 1982; 217:1263–5.
4. Segal S, Thier SO. Cystinuria. In: Scriver CR, Beaudet AL, Sly WS, Valle D, eds. The metabolic and molecular
bases of inherited disease, 7th ed. New York: McGraw-Hill, 1995:3581–3601.
5. Gahl WA, Ingelfinger J, Mohan P, Bernardini I, Hyman PE, Tangerman A. Intravenous cysteamine therapy for
nephropathic cystinosis. Pediatr Res 1995;38:579–84.
6. Hautmann R, Terhorst B, Stuhlsatz HW, Lutzeyer W. Mercaptopropionylglycine: a progress in cystine stone therapy.
J Urol 1977; 117:628.
7. Gerritsen T, Vaughn JG, Weisman HA. The identification of homocysteine in the urine. Biochem Biophys Res
Commun 1962;9: 493–6.
8. Chu RC, Hall CA. The total serum homocysteine as an indicator of vitamin B12 and folate status.Am J Clin Pathol
1988;90:446–9.
9. Kang S-S, Wong PWK, Malinow MR. Hyperhomocystinemia as a risk factor for occlusive vascular disease. Ann Rev
Nutr 1992;12:279–98.
10. Richie JP Jr, Skowronski L, Abraham P, Leutzinger Y. Blood Clinical Chemistry 44, No. 4, 1998 831 glutathione
concentrations in a large-scale human study. Clin Chem 1996;42:64–70. |
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GSH
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Product Code |
Unit |
Price |
Availability |
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5524 |
200 assay |
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