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 HOME > Cell Biology > Cell signaling |
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Aldehyde Quantitation Kit |
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코 드 |
: 10051 |
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단 위 |
: 200 assay |
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공 급 원 |
: AAT Bioquest |
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가 격 |
: 문의 |
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다운로드 |
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Aldehyde Assay Kit
Amplite™ Colorimetric Aldehyde Quantitation Kit
Very reactive aldehydes, namely 4-hydroxyalkenals, were first shown to be formed in autoxidizing chemical systems. It was subsequently shown that 4-hydroxyalkenals, particularly 4-hydroxynonenal, were formed in substantial amounts under biological conditions, i.e. during the peroxidation of lipids of liver microsomes incubated in the NADPH-Fe system. Many other aldehydes were also identified in peroxidizing liver microsomes or hepatocytes, e.g., alkanals , alk-2-enals, and 4-hydroxyalkenals.
Amplite™ Colorimetric Aldehyde Quantitation kit uses a proprietary dye that generates a chromogenic product upon reacting with an aldehyde. The kit provides a sensitive, one-step colorimetric method to detect as little as 1 nanomole of aldehyde in a 100 μL assay volume (10 μM). The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and readily adapted to automation without a separation step. Its signal can be easily read with an absorbance microplate reader at 405 or 550 nm.
Key Features
Application : Can be used for quantifying aldehydes in a variety of applications such as carbohydrate, lipid chemistry,
as well as enzyme reactions.
Sensitive : Detect as low as 1 nanomole of aldehyde.
Continuous : Easily adapted to automation without a separation step.
Convenient : Formulated to have minimal hands-on time.
Non-Radioactive : No special requirements for waste treatment.
Kit Components
Component A : AldeView™ Yellow 2 bottles
Component B : Assay Solution 1 bottle (10 mL)
Component C : Aldehyde Standard 1 vial
Component D : Dilution Buffer 1 bottle (20 mL)
Brief Summary
→ Prepare Enzyme reaction (50 μL)
→ Add 2X AldeView™ Yellow reaction mixture (50 μL)
→ Incubate at room temperature for 30-60 min
→ Read Absorbence at 405 or 550 nm
References
1. Trevor M. Kitson. (1985) High concentrations of aldehydes slow the reaction of cytoplasmic aldehyde
dehydrogenase with thiol-group modifiers Biochem. J. 228, 765.
2. Crabb DW, Matsumoto M, Chang D, You M (2004). Overview of the role of alcohol dehydrogenase and aldehyde
dehydrogenase and their variants in the genesis of alcohol-related pathology. The Proceedings of the Nutrition
Society 63 (1): 49.
3. Steinmetz CG, Xie P, Weiner H, Hurley TD (1997). Structure of mitochondrial aldehyde dehydrogenase: the genetic
component of ethanol aversion. Structure 5 (5): 701.
4. O'Donnell JM, Kudej RK, LaNoue KF, Vatner SF, Lewandowski ED. (2004) Limited transfer of cytosolic NADH into
mitochondria at high cardiac workload. Am J Physiol Heart Circ Physiol, 286, H2237.
5. Zurek G, and karst U (2000). 2,4-Dinitro-3,5,6-trideuterophenylhydrazones for the quantitation of aldehydes and
ketones in air samples by liquid chromatography-mass spectrometry. J of chromatography A, 869, 251.
6. Ou Z, Ogamo A, Guo L, Konda Y, Harigaya Y, and Nakagawa Y. (1995). Identification and quantitation of choline
glycerophospholipids that contain aldehyde residues by fluometric high-performance liquid chromatography.
Analytical biochemistry 227, 289. |
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10051
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Product Code |
Unit |
Fluorescene |
Availability |
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10051 |
200 assay |
Colorimetric
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10052
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200 assay |
Fluorimetric
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10053
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200 assay |
Colorimetric
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